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Recombinant EpCAM / CD326 (Epithelial Marker) Antibody [EGP40/8774R]

Additional information

Catalog Number: 4072-RBM39
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4072-RBM39-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4072-RBM39-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4072-RBM39-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
EpCAM Antibody in Flow Cytometry (FC)

EpCAM Antibody in Flow Cytometry (FC)

Flow Cytometric Analysis of CAPAN-2 cells using EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/9774R). Goat anti-Rabbit IgG-CF488 (red); Isotype Control (blue).

EpCAM Antibody in SDS-PAGE

EpCAM Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/8774R). Confirmation of Purity and Integrity of Antibody.

EpCAM Antibody in Immunohistochemistry (IHC (P))

EpCAM Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/8774R). Inset: PBS instead of primary antibody; secondary only negative control. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

  • IHC
  • WB
  • Flow
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot, Flow
Species Reactivity
Human
Clone
EGP40/8774R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell junction, Lateral cell membrane, Tight junction
Gene Name
EPCAM
Positive Control
HT29 cells. Human breast tumor.
Immunogen
Recombinant full-length human EpCAM protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Additional information

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Reactivity:

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Host:

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Additional information

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Formalin-fixed, paraffin-embedded Human Renal Cell Carcinoma Stained with KSP-Cadherin Rabbit Polyclonal Antibody.
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  • IHC

Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-RBP3
Reactivity:

, ,

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).
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  • IHC
  • WB

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Additional information

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Reactivity:

, , , ,

Gene:

Host:

VIEW ALL ANTIBODIES

Other Information

Functions
  • May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4072
SwissProt
P16422
Chromosome Location
2p21
Mol. Weight of Antigen
34.93kDa
Alternate Names
Epithelial cell adhesion molecule, Ber-Ep4|BerEp4|DIAR5|EGP-2|EGP314|EGP40|ESA|HNPCC8|KS1/4|KSA|LYNCH8|M4S1|MIC18|MK-1|MOC-31|TACSTD1|TROP1

Summary

Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM.EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

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