Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with Pan-Cytokeratin Mouse Monoclonal Antibody (PAN-CK).

Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with Pan-Cytokeratin Mouse Monoclonal Antibody (PAN-CK).

Confocal immunofluorescent analysis of HeLa cells using Pan-Cytokeratin Mouse Monoclonal Antibody (PAN-CK) followed by goat anti-mouse IgG-CF488 (cyan). Nuclei are counterstained with NucSpot (red).

Flow cytometric analysis of human HeLa cells. Pan-Cytokeratin Mouse Monoclonal Antibody (PAN-CK) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Printable Datasheet

Cytokeratin, pan (Epithelial Marker) [Cocktail PAN-CK]

Name: Cytokeratin, pan (Epithelial Marker)
SKU: MSM17-999
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 55kDa (CK7); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 46kDa (CK17); 45kDa (CK18) and 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad-spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.

Product Properties & Targets

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Species Reactivity

Cat, Chicken, Cow, Dog, Human, Monkey, Mouse, Rabbit, Rat

Isotype

IgG

Light Chain

Kappa

Gene Name

KRT77

Positive Control

Adeno- or Squamous carcinomas, HeLa, MCF-7, Skin

Immunogen

Human epidermal keratins

Alternate Names

Keratin, type II cytoskeletal 1b, Cytokeratin-1B, Keratin-77, Type-II keratin Kb39, K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39

Database Links

Entrez Gene ID

374454 (KRT77) & 51350 (KRT76)

Unigene

334989 (KRT77) & 654392 (KRT76)

SwissProt

Q7Z794 (KRT77) & Q01546 (KRT76)

Additional Information

Clone

Cocktail PAN-CK

Human Gene Symbol

KRT

Chromosome Location

17q21.2 (KRT77) & 12q13.13 (KRT76)

Mol. Weight of Antigen

40-67kDa (Multiple)

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (0.5-2ug/ml for 2 hours at RT)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8.
Tseng SCG et. al. Cell 1982; 30361.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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