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Recombinant Cytokeratin, pan (Epithelial Marker) Antibody [KRTL/1577R]

Additional information

Catalog Number: RBM21-1577
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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Recombinant Cytokeratin, pan (Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CK LMW Rabbit Recombinant Monoclonal Antibody (KRTL/1577R).

Cytokeratin, Acidic (Type I or LMW) Antibody in Western Blot (WB)

Cytokeratin, Acidic (Type I or LMW) Antibody in Western Blot (WB)

Western Blot analysis of Human Skin and A431 lysates using Cytokeratin, Acidic (Type I or LMW) Recombinant Rabbit Monoclonal Antibody (KRTL/1577R).

Cytokeratin Antibody in Western Blot (WB)

Cytokeratin Antibody in Western Blot (WB)

Western blot analysis of Human Skin, Mouse Skin, Rat Skin, Hamster Skin, Guinea pig Skin and Rabbit Skin tissue lysates using Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRTL/1577R).

KRT77 Antibody in SDS-PAGE

KRT77 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Keratin, type II cytoskeletal 1b Recombinant Rabbit Monoclonal Antibody (KRTL/1577R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
In Stock
Catalog Number Formulation Availability Size Price
RBM21-1577-P0
Purified Ab with BSA and Azide at 200ug/ml
Trial Size
In Stock
20ug
$259.00
Trial Size Available In-Stock
RBM21-1577-P1
Purified Ab with BSA and Azide at 200ug/ml
In Stock
100ug
$559.00
In-Stock
RBM21-1577-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
Carrier-Free
In Stock
100ug
$559.00
Carrier-Free Available In-Stock
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
KRTL/1577R
Isotype
IgG
Light Chain
Kappa
Gene Name
KRT77
Positive Control
Skin, Squamous cell carcinoma (SCC). A431.
Immunogen
Human epidermal keratin.
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
374454
SwissProt
Q7Z794
Chromosome Location
12q13.13
Mol. Weight of Antigen
61.9kDa
Alternate Names
Keratin, type II cytoskeletal 1b, K1B|KRT1B

Summary

This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50′, 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

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