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Recombinant Cytokeratin, pan (Epithelial Marker) Antibody [KRTL/7784R]

Additional information

Catalog Number: RBM54-7784
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
RBM54-7784-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
RBM54-7784-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
RBM54-7784-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin, pan (Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CK Type I Recombinant Rabbit Monoclonal Antibody (KRTL/7784R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Cytokeratin, Type I Antibody in Western Blot (WB)

Cytokeratin, Type I Antibody in Western Blot (WB)

Western Blot Analysis of Human Lung and A431 lysates using Cytokeratin, Type I Recombinant Rabbit Monoclonal Antibody (KRTL/7784R).

Cytokeratin, Type I Antibody in Western Blot (WB)

Cytokeratin, Type I Antibody in Western Blot (WB)

Western Blot Analysis of Lung tissue lysates of different species using Cytokeratin, Type I Recombinant Rabbit Monoclonal Antibody (KRTL/7784R).

KRT77 Antibody in SDS-PAGE

KRT77 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Keratin, type II cytoskeletal 1b Recombinant Rabbit Monoclonal Antibody (KRTL/7784R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
KRTL/7784R
Isotype
IgG
Light Chain
Kappa
Gene Name
KRT77
Positive Control
skin or squamous cell carcinoma. Lung, A431
Immunogen
Recombinant fragment (around aa100-200) of the human Cytokeratin, Type I protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Host:

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Other Information

PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
374454
SwissProt
Q7Z794
Chromosome Location
12q13.13
Mol. Weight of Antigen
61.9kDa
Alternate Names
Keratin, type II cytoskeletal 1b, K1B|KRT1B

Summary

The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation.This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. It usually used in a cytokeratin cocktail with type II cytokeratin antibody.

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