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Cytokeratin, pan (Epithelial Marker) Antibody [KRTL/1077 + KRTH/1076]

Additional information

Catalog Number: MSM10-1100
Gene:

Reactivity:

,

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
MSM10-1100-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
MSM10-1100-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
MSM10-1100-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin, pan (Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Cytokeratin, pan Monoclonal Antibody cocktail (KRTL/1077 + KRTH/1076).

Cytokeratin, pan (Epithelial Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded Rat Stomach stained with Cytokeratin, pan Monoclonal Antibody cocktail (KRTL/1077 + KRTH/1076).

Cytokeratin, pan (Epithelial Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded Rat Oviduct with Cytokeratin, pan Monoclonal Antibody cocktail (KRTL/1077 + KRTH/1076).

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human, Rat
Clone
KRTL/1077 + KRTH/1076
Isotype
IgG1
Light Chain
Kappa
Gene Name
KRT77
Positive Control
Adeno- or Squamous carcinomas., Skin
Immunogen
Recombinant human KRT77 and KRT76 protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Key References
  • Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8.
  • Tseng SCG et. al. Cell 1982; 30361.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
374454; 51350
SwissProt
Q7Z794 ; Q01546
Chromosome Location
17q21.2 (KRT77) & 12q13.13 (KRT76)
Mol. Weight of Antigen
67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19)
Alternate Names
Keratin, type II cytoskeletal 1b, Cytokeratin-1B, Keratin-77, Type-II keratin Kb39

Summary

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRTL/KRTH is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

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