Trial Size Available
Carrier-Free Available
Formalin-fixed, paraffin-embedded human placenta stained with CK, LMW Recombinant Mouse Monoclonal Antibody (rKRTL/8751). Inset: PBS instead of primary antibody; secondary only negative control.
SDS-PAGE Analysis of Purified CK LMW Recombinant Mouse Monoclonal (rKRTL/8751). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human Skin and A431 lysates using Cytokeratin, Acidic (Type I or LMW) Mouse Recombinant Monoclonal Antibody (rKRTL/8751).
Western blot analysis of skin tissue lysates from human, mouse, rat, hamster, rabbit and guinea pig using Cytokeratin, Type I Recombinant Mouse Monoclonal Antibody (rKRTL/8751).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
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