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Recombinant Cyclin E (G1/S-Phase Cyclin) Antibody [r13A3]

In Stock
Catalog Number Formulation Size Price
898-MSM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
898-MSM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
898-MSM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cyclin E Antibody in Immunohistochemistry (IHC (P))

Cyclin E Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human placenta stained with Cyclin E Recombinant Rabbit Mouse Antibody (r13A3). Inset: PBS instead of primary antibody; secondary only negative control.

Cyclin E Antibody in SDS-PAGE

Cyclin E Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Cyclin E Recombinant Mouse Monoclonal Antibody (r13A3). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Cyclin E was identified as a protein which would complement cyclin mutations in yeast and mammalian cells. Overexpression of cyclin E shortens the length of the G1 phase, accelerating progression of the cell cycle into S phase. The activity of cyclin E is mediated through its activation of cyclin dependent kinase 2 (cdk2) protein and is modulated by the presence of tumor suppressor proteins such as p16.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Infectious Disease, Lung Cancer, Nuclear Marker, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Nucleus
Gene Name
CCNE1
Positive Control
HeLa cells. Human breast or colon carcinoma.
Immunogen
Prokaryotic recombinant fusion protein corresponding to the full length cyclin E molecule
Alternate Names
G1/S-specific cyclin-E1, CCNE1; Cyclin E1; G1/S-specific cyclin-E1

Database Links

Entrez Gene ID
898
SwissProt
P24864

Additional Information

Clone
r13A3
Chromosome Location
19q12
Mol. Weight of Antigen
53kDa

Related Antibodies

View All CCNE1 Antibodies

Functions

  • Essential for the control of the cell cycle at the G1/S (start) transition.

Key References

  • Camacho F I, Garcia J F, Sanchez-Verde L, et al. American Journal of Pathology. 158 (4): 1363-1369 (2001).
  • Hayashi H, Ito T, Yazawa T, et al. Journal of Pathology. 192: 26–31 (2000). Weaver E J, Kovatich A J and Bibbo M. Acta Cytol. 44 (3): 301–304 (2000).
  • Ohtsubo M, Theodoras A M, Schumacher J, et al. Molecular and Cellular Biology. 15: 2612–2624 (1995).
  • Sewing A, Rönicke V, Bürger C, et al. Journal of Cell Science. 107: 581–588 (1994).
  • Keyomarsi K and Pardee A B. Proceedings of the National Academy of Sciences USA. 90; 1112–1116 (1993).
  • Dulic V, Lees E and Reed S I. Science. 257: 1958–1961 (1992).
  • Koff A, Cross F, Fisher A, et al. Cell. 66: 1217–1228 (1991).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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