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Carrier-Free Available
Formalin-fixed, paraffin-embedded human Pheochromocytoma stained with Chromogranin A Mouse Monoclonal Antibody (CHGA/777)
Western Blot of human Panc-1 cell lysate Chromogranin A Mouse Monoclonal Antibody (CHGA/777)
Formalin-fixed, paraffin-embedded human Pancreas stained with Chromogranin A Mouse Monoclonal Antibody (CHGA/777)
Formalin-fixed, paraffin-embedded human Adrenal Gland stained with Chromogranin A Mouse Monoclonal Antibody (CHGA/777)
SDS-PAGE Analysis of Purified CHGA Mouse Monoclonal Antibody(CHGA/777). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
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