Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CDX2 Mouse Monoclonal Antibody (CDX2/2214).

SDS-PAGE Analysis Purified CDX2 Mouse Monoclonal Antibody (CDX2/2214). Confirmation of Purity and Integrity of Antibody.

Analysis of Protein Array containing more than 19,000 full-length human proteins using CDX2 Mouse Monoclonal Antibody (CDX2/2214). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Printable Datasheet

CDX2 / Caudal Type Homeobox 2 (GI Epithelial Marker) [CDX2/2214]

Name: CDX2 / Caudal Type Homeobox 2 (GI Epithelial Marker)
SKU: 1045-MSM4
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoidsand is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma.However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.

Product Properties & Targets

Host

Mouse

Applications

Immunohistochemistry

Species Reactivity

Human

Isotype

IgG1

Cellular Localization

Nucleus

Light Chain

Kappa

Gene Name

CDX2

Positive Control

HT29 cells. Colon Carcinoma.

Immunogen

Recombinant human full-length CDX2 protein

Alternate Names

Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2, Caudal type homeobox 2; Caudal type homeobox transcription factor 2; Caudal-type homeobox protein 2; CDX2

Database Links

Entrez Gene ID

1045

Unigene

174249

SwissProt

Q99626

Additional Information

Clone

CDX2/2214

Human Gene Symbol

CDX2

Chromosome Location

13q12.2

Mol. Weight of Antigen

40kDa

Functions

Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity). Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity). Binds to and activates transcription of LPH (By similarity). Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity). Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed:28473536).

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95&degC followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Key References

Mallo, G.V., et al. 1998. J. Biol. Chem. 273: 14030-14036.
Silberg, D.G., et al. 2000. Gastroenterology 119: 961-971.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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