Recombinant CD7 (T-Cell Leukemia Marker) [CD7/8496R]

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Summary

Recognizes a protein of 40kDa, identified as CD7 (also known as gp40, Leu9). CD7 is a member of the immunoglobulin gene superfamily. Its N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
Cell surface
Gene Name
CD7
Positive Control
HePG-2 or Jurkat cells. Human lymph node or tonsil.
Immunogen
Recombinant fragment (around aa 72-175) of human CD7 protein (exact sequence is proprietary)
Alternate Names
T-cell antigen CD7, GP40, T-cell leukemia antigen, T-cell surface antigen Leu-9, TP41, GP40; Leu9; p41; T-cell leukemia antigen; T-cell surface antigen Leu-9; Tp40; TP41

Database Links

Entrez Gene ID
924
Unigene
SwissProt

Additional Information

Clone
CD7/8496R
Human Gene Symbol
CD7
Chromosome Location
17q25.3
Mol. Weight of Antigen
40kDa

Functions

  • Not yet known.

Known Applications & Suggested Dilutions

  • Western Blot (1-2ug/ml)
  • ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Saxena A, et al. Biologic and clinical significance of CD7 expression in acute myeloid leukemia. Am J Hematol. 1998;58(4):278-84.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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