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CD63 (Late Endosomes Marker) Antibody [NKI/C3]

Additional information

Catalog Number: 967-MSM1
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
967-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
967-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
967-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD63 Antibody in Immunofluorescence (IF)

CD63 Antibody in Immunofluorescence (IF)

Immunofluorescent Analysis of HeLa cells using CF488-labeled CD63 Mouse Monoclonal Antibody (NKI/C3). F-actin filaments are labeled with Dylight 554 phalloidin (red). DAPI was used to stain the cell nucleus (blue).

CD63 Antibody in Immunohistochemistry (IHC (P))

CD63 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded mouse spleen stained with CD63 Mouse Monoclonal Antibody (NKI/C3) (DAB Chromogen)

CD63 Antibody in Immunohistochemistry (IHC (P))

CD63 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human melanoma stained with CD63 Mouse Monoclonal Antibody (NKI/C3) (AEC Chromogen)

CD63 Antibody in Flow Cytometry (FC)

CD63 Antibody in Flow Cytometry (FC)

Flow Cytometric Analysis of PFA-fixed U87MG cells. CD63 Mouse Monoclonal Antibody (NKI/C3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

CD63 Antibody in SDS-PAGE

CD63 Antibody in SDS-PAGE

SDS-PAGE Analysis Purified CD63 Mouse Monoclonal Antibody (NKI/C3). Confirmation of Purity and Integrity of Antibody.

CD63 Antibody in Flow Cytometry (FC)

CD63 Antibody in Flow Cytometry (FC)

Flow cytometric analysis of human CD63 on MCF-7 cells. Black: cells alone; Green: isotype control; Red: PE-labeled CD63 Mouse Monoclonal Antibody (NKI/C3).

CD63 Antibody in Flow Cytometry (FC)

CD63 Antibody in Flow Cytometry (FC)

Flow cytometry analysis of live MCF-7 cells. Unstained (gray) or stained with biotin-labeled CD63 monoclonal antibody (NKI-C3) followed by streptavidin-CF568 (orange).

CD63 Antibody in Western Blot (WB)

CD63 Antibody in Western Blot (WB)

Western Blot Analysis of human lung tissue lysate using CD63 Mouse Monoclonal Antibody (NKI-C3)

CD63 Antibody in Western Blot (WB)

CD63 Antibody in Western Blot (WB)

Western Blot Analysis of lung tissue lysates of different species using CD63 Mouse Monoclonal Antibody (NKI-C3)

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Mouse
Clone
NKI/C3
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane, Cell surface, Cytoplasm, Endosome, Extracellular exosome, Late endosome membrane, Lysosome membrane, Melanosome, Multivesicular body, Secreted
Gene Name
CD63
Positive Control
SK-MEL-28, UM87MG or MCF-7 cells. Human melanoma or spleen. Lung.
Immunogen
Smooth plasma membrane fraction of MeWo cells
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Formalin-fixed, paraffin-embedded Mouse Heart stained with N-Cadherin Mouse Monoclonal Antibody (CDH2/1573).
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Formalin-fixed, paraffin-embedded mouse heart stained with N-Cadherin Mouse Monoclonal Antibody (8C11).
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SDS-PAGE Analysis of Purified N-Cadherin Mouse Monoclonal Antibody (13A9). Confirmation of Integrity and Purity of Antibody.
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Formalin-fixed, paraffin-embedded human kidney stained with MICA Mouse Recombinant Monoclonal Antibody (MICA/4442). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
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Flow cytometric analysis of PFA-fixed HeLa cells. HDAC6 Mouse Monoclonal Antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
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VIEW ALL ANTIBODIES

Other Information

Functions
  • Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
Key References
  • AA Palmer et al., Pathology 17: 335-339, 1985.
  • EC Hagen et al., Histopathology 10: 689-700, 1986.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
967
SwissProt
P08962
Chromosome Location
12q13.2
Mol. Weight of Antigen
26kDa (core protein); 30-60kDa (glycosylated)
Alternate Names
CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; melanoma 1 antigen; Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; MLA1; NGA; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Tetraspanin-30; TSPAN30

Summary

This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

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