Formalin-fixed, paraffin-embedded breast ductal carcinoma stained with Catenin, beta PAb. Note membrane staining in ductal carcinoma.

Formalin-fixed, paraffin-embedded breast lobular carcinoma stained with Catenin, beta PAb. Note cytoplasmic staining in lobular carcinoma.

Western Blot of Catenin, beta (p120) in human brain 1) absence and 2) presence of immunizing peptide and 3) Mouse brain lysate using Catenin, beta PAb.

Flow Cytometric Analysis of PFA fixed HeLa cells using Catenin, beta PAb followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Printable Datasheet

Catenin, beta (p120) []

Name: Catenin, beta (p120)
SKU: 1499-RBP1
Availability: InStock

Polyclonals Primary Antibodies Rabbit Polyclonals

Summary

Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Product Properties & Targets

Host

Rabbit

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Species Reactivity

Human, Mouse

Research Areas

Apoptosis, Autophagy, Cancer, Cardiovascular

Isotype

IgG

Cellular Localization

Adherens junction, Cell junction, Cell membrane, Centrosome, Cilium basal body, Cytoplasm, Cytoskeleton, Microtubule organizing center, Nucleus, Spindle pole, Synapse

Light Chain

Kappa

Gene Name

CTNNB1

Positive Control

HeLa or MCF-7 cells. Breast carcinoma.

Immunogen

A synthetic peptide from the middle of beta-Catenin (CTNNB1) protein

Alternate Names

Catenin beta-1, Beta-catenin, Cadherin associated protein), beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1

Database Links

Entrez Gene ID

1499

Unigene

476018

SwissProt

P35222

Additional Information

Human Gene Symbol

CTNNB1

Chromosome Location

3p22.1

Mol. Weight of Antigen

92kDa

Functions

Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed:18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity).

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Key References

Dabbs DJ et. al. Am J Surg Path. 2007;31:427-437.
Sarrio D et. al. Oncogene. 2004;23:3272-3283.
Mastracci TL et. al. Mod Path. 2005;18:741-751.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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