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Formalin-fixed, paraffin-embedded breast ductal carcinoma stained with Catenin, beta PAb. Note membrane staining in ductal carcinoma.
Formalin-fixed, paraffin-embedded breast lobular carcinoma stained with Catenin, beta PAb. Note cytoplasmic staining in lobular carcinoma.
Western Blot of Catenin, beta (p120) in human brain 1) absence and 2) presence of immunizing peptide and 3) Mouse brain lysate using Catenin, beta PAb.
Flow Cytometric Analysis of PFA fixed HeLa cells using Catenin, beta PAb followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).
SDS-PAGE Analysis of Purified Beta Catenin Mouse Monoclonal Antibody (Rabbit PAb). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
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