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Recombinant Catenin, beta (CTNNB1) Antibody [rCTNNB1/8043]

In Stock
Catalog Number Formulation Size Price
1499-MSM21-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
1499-MSM21-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
1499-MSM21-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Catenin, beta (CTNNB1) Antibody

Formalin-fixed, paraffin-embedded human skin stained with Beta-Catenin Mouse Recombinant Monoclonal Antibody (rCTNNB1/8043). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Catenin, beta (CTNNB1) Antibody - Image 2

Formalin-fixed, paraffin-embedded human prostate stained with Beta-Catenin Mouse Recombinant Monoclonal Antibody (rCTNNB1/8043). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Catenin, beta (CTNNB1) Antibody - Image 3

SDS-PAGE Analysis of Purified Beta-Catenin Mouse Recombinant Monoclonal (rCTNNB1/8043). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Breast Cancer, Cardiovascular, Developmental Biology, Immunology, Basal Cell Marker, BBB VCAM-1 Signaling, Colon Cancer, Infectious Disease, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell Surface. Cytoplasm.
Gene Name
CTNNB1
Positive Control
HeLa or MCF-7 cells. Human breast carcinoma
Immunogen
Recombinant fragment (around aa1-200) of human β-catenin protein (exact sequence is proprietary)
Alternate Names
Catenin beta-1, Beta-catenin, Cadherin associated protein, beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1

Database Links

Entrez Gene ID
1499
SwissProt
P35222Human

Additional Information

Clone
rCTNNB1/8043
Chromosome Location
3p22.1
Mol. Weight of Antigen
92kDa

Related Antibodies

View All CTNNB1 Antibodies

Functions

  • Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed:18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity).

Key References

  • Dabbs DJ et. al. Am J Surg Path. 2007;31:427-437.
  • Sarrio D et. al. Oncogene. 2004;23:3272-3283.
  • Mastracci TL et. al. Mod Path. 2005;18:741-751.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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