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Recombinant APC / Adenomatous Polyposis Coli / FAP (Tumor Suppressor) Antibody [rEMM43]

In Stock
Catalog Number Formulation Size Price
324-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
324-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
324-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Adenomatous Polyposis Coli Protein Antibody in Immunohistochemistry (IHC (P))

Adenomatous Polyposis Coli Protein Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human testis stained with APC Recombinant Mouse Monoclonal Antibody (rEMM43). Inset: PBS instead of primary antibody; secondary only negative control.

Adenomatous Polyposis Coli Protein Antibody in SDS-PAGE

Adenomatous Polyposis Coli Protein Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified APC Recombinant Mouse Monoclonal Antibody (rEMM43). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The human adenomatous polyposis coli (APC) gene at locus 5q21 encodes a protein of 2,843 amino acids. The precise function of this 300 kD protein is not clear but it may have several functions including a role in cell proliferation, apoptosis, expression of cell adhesion proteins
and modulation of cell to cell interactions. It is reported to be expressed in the cytoplasm of cell types of many lineages, both epithelial and mesenchymal. In the epithelium of bladder, small and large intestine, esophagus, stomach and epidermis, APC expression is restricted to regions in
which cell replication has ceased and terminal differentiation has been established. Expression has been observed in lung, liver, kidney and mammary gland endothelial, myoepithelial and duct lining epithelial cells. Some tissues such as ovary, myometrium, thyroid, parathyroid and tonsil do not express the protein.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Apoptosis, Breast Cancer, Cardiovascular, Colon Cancer, Infectious Disease, Ovarian Cancer, Signal Transduction
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Adherens junction, Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Lamellipodium, Ruffle membrane
Gene Name
APC
Positive Control
Human tonsil, placenta, breast or colon. HCT116 or COLO 320DM cells.
Immunogen
Prokaryotic recombinant protein corresponding to a 251 amino acid region of the C-terminus of the human adenomatous polyposis coli protein
Alternate Names
Adenomatous polyposis coli protein, Deleted in polyposis 2.5, Adenomatous Polyposis Coli; CC1; Deleted in polyposis 2.5 (DP2.5); DP2; DP3; FAP; FPC; GS; Protein APC

Database Links

Entrez Gene ID
324
SwissProt
P25054

Additional Information

Clone
rEMM43
Chromosome Location
5q22.2
Mol. Weight of Antigen
66kDa

Related Antibodies

View All APC Antibodies

Functions

  • Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Key References

  • Sunkel, C.E. and Glover, D.M. 1988. J. Cell Sci. 89: 25-38.
  • Furuuchi K, Tada M, Yamada H, et al. American Journal of Pathology. 156 (6): 1997–2005 (2000).
  • Ho K Y K, Kalle W H J, Lo T H S, et al. Histopathology. 35: 249–256 (1999).
  • Midgley C A, White S, Howitt R, et al.. Journal of Pathology. 181: 426–433 (1997).
  • Smith K J, Johnson K A, Bryan T M, et al. Proceedings of the National Academy of Sciences USA. 90: 2846–2850 (1993).
  • Groden J, Thliveris A, Samowitz W, et al. Cell. 66: 589–600 (1991).
  • Nishisho I, Nakamura Y, Miyoshi Y, et al. Science. 253: 665–669 (1991).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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