Home >> Antibodies >> von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker)

von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker)

Mouse Monoclonal Antibody [Clone IIIE2.34]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

199.00

459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00

459.00

Printable

Key References

Bioinformatics

Product Details

Synonyms

Coagulation Factor VIII, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

Positive Control

HUVEC cells. Human tonsil.

Known Applications & Suggested Dilutions

IP, Flow Cyt, IF, Western Blot, IHC-P More Details

Immunoprecipitation (0.5-1 g/500ug protein lysate)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human vWF fragment spanning around aa 845-949 (exact sequence is proprietary)

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

250kDa

Specificity & Comments

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.