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Home >> Antibodies >> von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker)

von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker)

Mouse Monoclonal Antibody [Clone 3E2D10 + VWF635]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human uterus stained with vWF Mouse Monoclonal Antibody (3E2D10 + VWF635)

Product Details

Synonyms

Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

Positive Control

HUVEC cells. Human tonsil.

Known Applications & Suggested Dilutions

IP, Flow Cyt, IF, Western Blot, IHC-P         More Details

Immunoprecipitation (1-2 g/500ug protein lysate)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human vWF fragment spanning around aa 845-949 (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa (3E2D10) + Mouse / IgG1, kappa (VWF635)

Mol. Weight of Antigen

250kDa

Specificity & Comments

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Motta, A.et al.2009. J Biomater Sci Polym Ed. 20: 1875-1897.
  2. Germann, B.et al.2008. Pharmazie. 63: 303-307.

Bioinformatics

  •  Entrez Gene ID: 7450
  •  Gene Symbol: VWF
  •  SwissProt: P04275
  •  Chromosome Location: 12p13.31
  •  Unigene: 440848