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Home >> Antibodies >> Vimentin (Mesenchymal Cell Marker)

Vimentin (Mesenchymal Cell Marker)

Mouse Monoclonal Antibody [Clone LN-6]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).

Product Details

Synonyms

VIM

Positive Control

Jurkat cells, Sarcomas, Melanomas.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human thymic nuclear extract

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Cat. Cow. Pig. Rat. Rabbit. Mouse. Sheep.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

57-60kDa

Specificity & Comments

This MAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Stathopoulos, E., et al., J. Histochem. Cytochem. 37, 1363 (1989).

Bioinformatics

  •  Entrez Gene ID: 7431
  •  Gene Symbol: VIM
  •  SwissProt: P08670
  •  Chromosome Location: 10p12.33
  •  Unigene: 455493
NBT00060