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Home >> Antibodies >> Villin (GI-Mucosal & Urogenital Brush Border Marker)

Villin (GI-Mucosal & Urogenital Brush Border Marker)

Mouse Monoclonal Antibody [Clone VIL1/1325]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Rectum stained with Villin Mouse Monoclonal Antibody (VIL1/1325).

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Formalin-fixed, paraffin-embedded human Colon stained with Villin Mouse Monoclonal Antibody (VIL1/1325).

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Western Blot of A549, HepG2 & HCT116 cell lysates Villin Mouse Monoclonal Antibody (VIL1/1325).

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SDS-PAGE Analysis Purified Villin Mouse Monoclonal Antibody (VIL1/1325). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

VIL1; Villin-1; Villin1

Positive Control

A549, HepG2 and HCT116 cells. Colon or Rectum.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml for 60 minutes at RT)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary))

Cellular Localization

Cytoplasmic & Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

93kDa

Specificity & Comments

Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. West BA, Isaac CA, Carboni JM, Morrow JS, Mooseker MS, Barwick KW. Localization of villin, a cytoskeletal protein specific to microvilli, in human ileum and colon and in colonic neoplasms. Gastroenterology. 1988;94:343-52.
  2. Bacchi CE, Gown AM. Distribution and pattern of expression of villin, a gastrointestinal-associated cytoskeletal protein, in human carcinomas: a study employing paraffin-embedded tissue. Lab Invest. 1991;64:418-24.

Bioinformatics

  •  Entrez Gene ID: 7429
  •  Gene Symbol: VIL1
  •  SwissProt: P09327
  •  Chromosome Location: 2q35
  •  Unigene: 654595
NBT01325