Home >> Antibodies >> Villin (GI-Mucosal & Urogenital Brush Border Marker)

Villin (GI-Mucosal & Urogenital Brush Border Marker)

Mouse Monoclonal Antibody [Clone VIL1/1314 + VIL1/2376]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

199.00

459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00

459.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Small Intestine stained with Villin Mouse Monoclonal Antibody (VIL1/1314 + VIL1/2376).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Villin Mouse Monoclonal Antibody (VIL1/1314 + VIL1/2376).

Formalin-fixed, paraffin-embedded human Small Intestine stained with Villin Mouse Monoclonal Antibody (VIL1/1314 + VIL1/2376).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Villin Mouse Monoclonal Antibody (VIL1/1314 + VIL1/2376).

Product Details

Synonyms

VIL1; Villin-1; Villin1

Positive Control

A549, HepG2 and HCT116 cells. Colon or Rectum.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A recombinant fragment (around aa179-311) of human Villin protein (exact sequence is proprietary)

Cellular Localization

Cytoplasmic & Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa + Mouse / IgG1, kappa

Mol. Weight of Antigen

93kDa

Specificity & Comments

Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.