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Home >> Antibodies >> UBE2B

UBE2B

Mouse Monoclonal Antibody [Clone PCRP-UBE2B-1C7]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed HeLa cells.UBE2B Mouse Monoclonal Antibody (PCRP-UBE2B-1C7) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescent Analysis of PFA-fixed HeLa cells. UBE2B Mouse Monoclonal Antibody (PCRP-UBE2B-1C7) followed by IgG-CF488 (green), counterstained with phalloidin.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using UBE2B Mouse Monoclonal Antibody (PCRP-UBE2B-1C7).

Product Details

Synonyms

E2 17kDa; hHR6B; HR6B; RAD6 homolog B (RAD6B); UBC2; UBE2B; Ubiquitin carrier protein B; Ubiquitin conjugating enzyme E2B; Ubiquitin protein ligase B; Ubiquitin-conjugating enzyme E2 17 kDa;

Positive Control

HeLa, K562 or Jurkat cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human UBE2B protein

Cellular Localization

Cell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.

Species Reactivity

Human. Predicted to work in Mouse, Rat, Xenopus and Zebrafish.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

17kDa

Specificity & Comments

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (ubiquitin-conjugating enzyme E2 A) and UBE2B (ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to post replication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ciechanover, A. 1994. The ubiquitin-proteasome proteolytic pathway. Cell 79: 13-21.
  2. Hochstrasser, M. 1995. Ubiquitin, proteasomes and the regulation of intracellular protein degradation. Curr. Opin. Cell Biol. 7: 215-223.

Bioinformatics