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UBE2B Antibody [PCRP-UBE2B-1C7]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
7320-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7320-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7320-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml

Summary

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (ubiquitin-conjugating enzyme E2 A) and UBE2B (ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to post replication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2b /
Cellular Localization
Cell membrane, Nucleus
Gene Name
Positive Control
HeLa, K562 or Jurkat cells.
Immunogen
Recombinant full-length human UBE2B protein
Alternate Names
Ubiquitin-conjugating enzyme E2 B, E2 ubiquitin-conjugating enzyme B, RAD6 homolog B, Ubiquitin carrier protein B, Ubiquitin-conjugating enzyme E2-17 kDa, Ubiquitin-protein ligase B, E2 17kDa; hHR6B; HR6B; RAD6 homolog B (RAD6B); UBC2; UBE2B; Ubiquitin carrier protein B; Ubiquitin conjugating enzyme E2B; Ubiquitin protein ligase B; Ubiquitin-conjugating enzyme E2 17 kDa;

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-UBE2B-1C7
Chromosome Location
5q31.1
Mol. Weight of Antigen
17kDa

Functions

  • Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.

Key References

  • Ciechanover, A. 1994. The ubiquitin-proteasome proteolytic pathway. Cell 79: 13-21.
  • Hochstrasser, M. 1995. Ubiquitin, proteasomes and the regulation of intracellular protein degradation. Curr. Opin. Cell Biol. 7: 215-223.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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