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Home >> Antibodies >> SOX10 (Melanoma Marker)

SOX10 (Melanoma Marker)

Mouse Monoclonal Antibody [Clone SOX10/1074]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Melanoma stained with SOX10 Mouse Monoclonal Antibody (SOX10/1074).

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Western Blot Analysis of SOX10 (A) Recombinant protein; (B) A375 cell lysate using SOX10 Mouse Monoclonal Antibody (SOX10/1074).

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Formalin-fixed, paraffin-embedded Mouse Brain stained with SOX10 Mouse Monoclonal Antibody (SOX10/1074).

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SDS-PAGE Analysis Purified SOX10 Mouse Monoclonal Antibody (SOX10/1074). Confirmation of Integrity and Purity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX10 Mouse Monoclonal Antibody (SOX10/1074)

Product Details

Synonyms

DOM; PCWH; SOX10; SRY (sex determining region Y) box 10; SRY box containing gene 10; SRY related HMG box gene 10; Waardenburg syndrome type 2E(WS2E); WS4; Waardenburg syndrome type 4C (WS4C)

Positive Control

HepG2 cells. Melanomas, breast carcinomas, gliomas.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human SOX10 protein fragment (around aa115-269) (exact sequence is proprietary)

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

49-58kDa

Specificity & Comments

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of ~55kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately-to-strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Mohamed A, et al. Appl Immunohistochem Mol Morphol. 2013; 21(6):506-10.

Bioinformatics

  •  Entrez Gene ID: 6663
  •  Gene Symbol: SOX10
  •  SwissProt: P56693
  •  Chromosome Location: 22q13.1
  •  Unigene: 376984
NBT01074