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Home >> Antibodies >> S100B (Astrocyte and Melanoma Marker)

S100B (Astrocyte and Melanoma Marker)

Mouse Monoclonal Antibody [Clone S100B/1012]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

459.00

Formulation

Purified Ab conjugated to CF488

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to CF488

Price (USD)

459.00
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Confocal Immunofluorescent analysis of A2058 cells using CF488-labeled S100B Monoclonal Antibody (S100B/1012) (Green). F-actin filaments were labeled with Dylight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).

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Confocal Immunofluorescent analysis of A2058 cells using CF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). F-actin filaments were labeled with Dylight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)

Product Details

Synonyms

NEF; Protein S100-B; S-100 protein beta chain; S100 calcium binding protein beta (neural); S100 calcium-binding protein B; S100 protein beta chain; S100B; S100beta

Positive Control

Brain, Melanoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human S100B protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse. Rat. Cow.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

10-12kDa

Specificity & Comments

S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

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Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Key References

  1. Joshi MG et. al. Modern Pathology, 1996, 9(1):57-62.
  2. McLaren KM et. al. Human Pathology, 1996, 27(7):633-6.

Bioinformatics

  •  Entrez Gene ID: 6285
  •  Gene Symbol: S100B
  •  SwissProt: P04271
  •  Chromosome Location: 21q22.3
  •  Unigene: 422181
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