Formalin-fixed, paraffin-embedded human Hepatic Carcinoma stained with Prohibitin Mouse Monoclonal Antibody (SPM311).
SDS-PAGE Analysis Purified Prohibitin Mouse Monoclonal Antibody (SPM311). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of human liver tissue lysateusing Prohibitin Mouse Monoclonal Antibody (SPM311).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a protein of 30kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although prohibitin mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. Prohibitin is an ideal mitochondrial marker. It shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.