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Home >> Antibodies >> PRMT6

PRMT6

Mouse Monoclonal Antibody [Clone PCRP-PRMT6-2C9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed HeLa cells. PRMT6 Mouse Monoclonal Antibody (PCRP-PRMT6-2C9) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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SDS-PAGE Analysis. Purified PRMT6 Mouse Monoclonal Antibody (PCRP-PRMT6-2C9). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using PRMT6 Mouse Monoclonal Antibody (PCRP-PRMT6-2C9) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

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SDS-PAGE Analysis. Purified PRMT6 Mouse Monoclonal Antibody (PCRP-PRMT6-2C9). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using PRMT6 Mouse Monoclonal Antibody (PCRP-PRMT6-2C9).

Product Details

Synonyms

Chromobox protein homolog 7; Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6; Histone-arginine N-methyltransferase PRMT6; HMT1 hnRNP methyltransferase like 6.; HRMT1L6

Positive Control

HeLa, MCF7 or HepG2 cells.

Known Applications & Suggested Dilutions

IP, Flow Cyt, IF         More Details

Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human PRMT6 protein

Cellular Localization

Nucleus.

Species Reactivity

Human. Predicted to react with Rat.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

42kDa

Specificity & Comments

A class of proteins termed type 1 protein arginine N-methyltransferase (PRMT) enzymes contribute to posttranslational modification of RNA-binding proteins, but differ in substrate specificities, oligomerization properties and subcellular localization. PRMTs contain an S-adenosylmethione motif which functions to add one or two methyl groups to guanidino nitrogens of arginine (R) side chains. PRMT6, also known as HRMT1L6, is a nuclear protein belonging to the PRMT family and is predominantly expressed in testis and kidney. It is known to methylate Histones H3, H4 and H2A. PRMT6 is the major dimethyltransferase for Histone H3 and specifically methylates Histone H3 at R2. Methylation at Histone H3 R2 acts to inhibit Histone H3 K4 trimethylation and ultimately leads to the transcriptional repression of genes that are activated by Histone H3 K4 trimethylation. In addition, PRMT6 methylates HIV TAT, possibly functioning as a form of cellular innate immunity to restrict levels of HIV replication.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Boulanger, M.C., et al. 2005. J. Virol. 79: 124-131.
  2. Blackshaw S., et al. Nature methods15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 55170
  •  Gene Symbol: PRMT6
  •  SwissProt: Q96LA8
  •  Chromosome Location: 1p13.3
  •  Unigene: 26006