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PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)

Recombinant Mouse Monoclonal Antibody [Clone rPDL1/4773]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human skin stained with PD-L1 Recombinant Mouse Monoclonal Antibody (rPDL1/4773).

Formalin-fixed, paraffin-embedded human placenta stained with PD-L1 Recombinant Mouse Monoclonal Antibody (rPDL1/4773).

SDS-PAGE Analysis Purified PD-L1 Recombinant Mouse Monoclonal Antibody (rPDL1/4773). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of NIH3T3, PC3 and MCF-7 cell lysates using PD-L1 Mouse Monoclonal Antibody (PDL1/2746).

Formalin-fixed, paraffin-embedded human skin stained with PD-L1 Recombinant Mouse Monoclonal Antibody (rPDL1/4773).

Formalin-fixed, paraffin-embedded human placenta stained with PD-L1 Recombinant Mouse Monoclonal Antibody (rPDL1/4773).

SDS-PAGE Analysis Purified PD-L1 Recombinant Mouse Monoclonal Antibody (rPDL1/4773). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of NIH3T3, PC3 and MCF-7 cell lysates using PD-L1 Mouse Monoclonal Antibody (PDL1/2746).

Product Details

Synonyms

B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1

Positive Control

NIH3T3, PC3 or MCF-7 cells. Human placenta, spleen or tonsil tissue.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

37-50kDa

Specificity & Comments

PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.