Home >> Antibodies >> PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)

PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)

Recombinant Rabbit Monoclonal Antibody [Clone PDL1/4451R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Lung SqCC stained with PD-L1 Recombinant Rabbit Monoclonal Antibody (PDL1/4451R).

Formalin-fixed, paraffin-embedded human Lung SqCC stained with PD-L1 Recombinant Rabbit Monoclonal Antibody (PDL1/4451R).

Western Blot Analysis of A431, MCF-7, JEG-3, MDA-MB231, SW732 cell lysates using PD-L1 Recombinant Rabbit Monoclonal Antibody (PDL1/4451R).

Formalin-fixed, paraffin-embedded human Lung SqCC stained with PD-L1 Recombinant Rabbit Monoclonal Antibody (PDL1/4451R).

Western Blot Analysis of A431, MCF-7, JEG-3, MDA-MB231, SW732 cell lysates using PD-L1 Recombinant Rabbit Monoclonal Antibody (PDL1/4451R).

Product Details

Synonyms

B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1

Positive Control

Human heart, placenta, spleen or tonsil tissue. Jurkat, RAW or HepG2 cells.

Known Applications & Suggested Dilutions

Western Blot, Flow Cyt, IF, IHC-P More Details

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human and Mouse.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

37-50kDa

Specificity & Comments

PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.