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Home >> Antibodies >> Pancreatic duodenal homeobox-1 protein (PDX1)

Pancreatic duodenal homeobox-1 protein (PDX1)

Mouse Monoclonal Antibody [Clone PCRP-PDX1-2C11]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed HeLa cells. PDX1 Mouse Monoclonal Antibody (PCRP-PDX1-2C11) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescence Analysis of PFA-fixed HeLa cells. PDX1 Mouse Monoclonal Antibody (PCRP-PDX1-2C11) followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

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SDS-PAGE Analysis Purified PDX1 Mouse Monoclonal Antibody (PCRP-PDX1-2C11). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using PDX1 Mouse Monoclonal Antibody (PCRP-PDX1-2C11).

Product Details

Synonyms

Glucose sensitive factor; GSF; IDX1; Insulin promoter factor 1 homeodomain transcription factor; IPF1; STF1; IUF1; Islet/duodenum homeobox 1; MODY4; Pancreas/duodenum homeobox; Somatostatin transactivating factor 1

Positive Control

HeLa or HEK293 cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human PDX1 protein

Cellular Localization

Nucleus.

Species Reactivity

Human

Host / Ig Isotype

Mouse / IgG2c

Mol. Weight of Antigen

46kDa

Specificity & Comments

Pancreatic duodenal homeobox-1 protein (PDX-1), also designated Insulin promoter factor (IPF1), Insulin upstream factor 1 (IUF1), somatostatin transactivating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic -cells. PDX-1 is a key regulator of pancreatic islet development and Insulin gene transcription in -cells. PDX-1 is expressed in all cells at the early stages of development and is mainly restricted to the pancreas and duodenum in adult. HNF-3, HNF-1 and SP1 positively regulate the PDX-1 enhancer element. PDX-1 is also regulated by Glucagon-like peptide through activation of adenylyl cyclase, which results in an increase in intracellular cAMP activity. The increased levels of cAMP, and the resulting activation of PKA, lead to an increase in PDX-1 transcription and translocation of the protein to the nuclei of -cells. PDX-1 binds to the sequence C(C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by the SAPK2 pathway under high glucose concentrations. Mutations in the PDX-1 gene can cause maturity-onset diabetes of the young and pancreatic agenesis. The gene which encodes PDX-1 maps to human chromosome 13q12.2.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Stoffel, M., et al. 1995. Genomics 28: 125-126.
  2. Blackshaw S., et al. Nature methods15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 3651
  •  Gene Symbol: PDX1
  •  SwissProt: P52945
  •  Chromosome Location: 13q12.2
  •  Unigene: 32938