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Home >> Antibodies >> p21WAF1 (Tumor Suppressor Protein)

p21WAF1 (Tumor Suppressor Protein)

Recombinant Rabbit Monoclonal Antibody [Clone CIP1/4377R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin fixed paraffin embedded human colon carcinoma stained with p21 Rabbit Recombinant Monoclonal Antibody (CIP1/4377R).

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IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using CIP1/4377R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

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Formalin fixed paraffin embedded human lung carcinoma stained with p21 Rabbit Recombinant Monoclonal Antibody (CIP1/4377R).

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Western Blot Analysis of HeLa cell lysate using p21 Rabbit Recombinant Monoclonal Antibody (CIP1/4377R).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using p21-Monospecific Rabbit Recombinant Monoclonal Antibody (CIP1/4377R).

Product Details

Synonyms

Activating Fragment 1, CAP20, CDK-interacting protein 1, CDKI, CDKN1, CDKN1A, CIP1, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), DNA Synthesis Inhibitor, MDA6, Melanoma Differentiation Associated Protein 6, PIC1, SDI1, SLC12A9, p21Cip1/Waf1, Wild type p53 activated fragment 1 (WAF1)

Positive Control

HeLa cells. Human skin, colon, or breast carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (2-4ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Synthetic peptide corresponding to p21 residues within aa1-100 of p21 was used as an immunogen.

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

21kDa

Specificity & Comments

This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Harper, J.W., et al. 1993. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816.

Bioinformatics

  •  Entrez Gene ID: 1026
  •  Gene Symbol: CDKN1A
  •  SwissProt: P38936
  •  Chromosome Location: 6p21.31
  •  Unigene: 370771