Formalin-fixed, paraffin-embedded human tonsil stained with Nucleolin Mouse Monoclonal Antibody (SPM614).
Immunofluorescence analysis of HeLa cells stained with CF647R-Nucleolin Mouse Monoclonal Antibody (SPM614) (green). Nuclei counterstained stained with DAPI (blue).
Immunofluorescence analysis of PFA-fixed MCF-7 cells. Nucleolin Mouse Monoclonal Antibody (SPM614) followed by goat anti-mouse IgG-CF488 (green). Membrane stained with phalloidin-CF640.
Immunofluorescence analysis of PFA-fixed K562 cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) followed by goat anti-mouse IgG-CF488 (green). Membrane stained with phalloidin-CF640.
Flow Cytometric Analysis of PFA-fixed K562 cells labeled with Nucleolin Mouse Monoclonal Antibody (SPM614) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.