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Home >> Antibodies >> Nucleolin (Marker of Human Cells)

Nucleolin (Marker of Human Cells)

Mouse Monoclonal Antibody [Clone SPM614]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Monoclonal Antibody (SPM614).

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Immunofluorescence Analysis of HeLa cells stained with CF647R labeled Nucleolin Mouse Monoclonal Antibody (SPM614)(Green) Blue: DAPI.

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Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Monoclonal Antibody (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.

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Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin MAb (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.

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Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin MAb (SPM614) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

NCL; Nucl; Nucleolin; Protein C23

Positive Control

All human cells. Testis, Ovary, Uterus, Endometrial or Hodgkin's lymphoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-4ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human NCL protein

Cellular Localization

Nucleoli

Species Reactivity

Human. Does not react with Mouse, Rat or Cow.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

76kDa

Specificity & Comments

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Fujiki H, Watanabe T, Suganuma M. Cell-surface nucleolin acts as a central mediator for carcinogenic, anti-carcinogenic, and disease-related ligands. J Cancer Res Clin Oncol. 2014;140(5):689-99.
  2. Qiu W, Zhou F, Zhang Q, Sun X, Shi X, Liang Y, Wang X, Yue L. Overexpression of nucleolin and different expression sites both related to the prognosis of gastric cancer. APMIS. 2013;121(10):919-25.

Bioinformatics

  •  Entrez Gene ID: 4691
  •  Gene Symbol: NCL
  •  SwissProt: P19338
  •  Chromosome Location: 2q37.1
  •  Unigene: 79110
NBT00902