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Nucleolin (Marker of Human Cells) Antibody [364-5 + NCL/902]

In Stock
Catalog Number Formulation Size Price
4691-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
4691-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
4691-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Nucleolin (Marker of Human Cells) Antibody

Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902) followed by goat anti-mouse IgG-CF488 (green). Membrane is stained with Phalloiden-CF640.

Nucleolin (Marker of Human Cells) Antibody - Image 2

Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902) followed by goat anti-mouse IgG-CF488 (green). Membrane is stained with Phalloiden-CF640.

Nucleolin (Marker of Human Cells) Antibody - Image 3

Formalin-fixed, paraffin-embedded human colon stained with AF488 labeled Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902).

Nucleolin (Marker of Human Cells) Antibody - Image 4

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902).

Nucleolin (Marker of Human Cells) Antibody - Image 5

Flow Cytometric Analysis of PFA-fixed K562 cells. Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Nucleolin (Marker of Human Cells) Antibody - Image 6

Formalin-fixed, paraffin-embedded human tonsil stained with Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902).

Nucleolin (Marker of Human Cells) Antibody - Image 7

Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902).

Nucleolin (Marker of Human Cells) Antibody - Image 8

Formalin-fixed, paraffin-embedded human uterus stained with Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902).

NCL Antibody in SDS-PAGE

NCL Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Nuclear Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Nucleolus, Nucleus
Gene Name
NCL
Positive Control
All human cells. Human testis, endometrial or Hodgkin s lymphoma., ovary, Uterus
Immunogen
Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902)
Alternate Names
Nucleolin, Protein C23, NCL; Nucl; Nucleolin; Protein C23

Database Links

Entrez Gene ID
4691
SwissProt
P19338

Additional Information

Clone
364-5 + NCL/902
Chromosome Location
2q37.1
Mol. Weight of Antigen
76kDa

Related Antibodies

View All NCL Antibodies

Functions

  • Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Key References

  • Fujiki H, Watanabe T, Suganuma M. Cell-surface nucleolin acts as a central mediator for carcinogenic, anti-carcinogenic, and disease-related ligands. J Cancer Res Clin Oncol.2014;140(5):689-99.
  • Qiu W,Zhou F,Zhang Q,Sun X,Shi X,Liang Y,Wang X,Yue L. Overexpression of nucleolin and different expression sites both related to the prognosis of gastric cancer. APMIS.2013;121(10):919-25.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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