Contact Us

Recombinant Nucleolin (Marker of Human Cells) Antibody [NCL/8695R]

Additional information

Catalog Number: 4691-RBM19
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

download data sheet
In Stock
Catalog Number Formulation Size Price
4691-RBM19-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4691-RBM19-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4691-RBM19-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Nucleolin (Marker of Human Cells) Antibody

SDS-PAGE Analysis of Purified Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/8695R). Confirmation of Integrity and Purity of Antibody.

Recombinant Nucleolin (Marker of Human Cells) Antibody - Image 2

Formalin-fixed, paraffin-embedded human skin stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/8695R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
NCL/8695R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
nucleoli
Gene Name
NCL
Positive Control
All human cells. Human testis|ovary or Hodgkin s lymphoma.
Immunogen
Recombinant full-length human NCL protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

SDS-PAGE Analysis of Purified CDH16 Rabbit Recombinant Monoclonal Antibody (CDH16/7028R). Confirmation of Purity and Integrity of Antibody.
4
  • IHC
  • WB

Recombinant Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-RBM11
Reactivity:

, ,

Gene:

Host:

Formalin-fixed, paraffin-embedded mouse heart stained with N-Cadherin Mouse Monoclonal Antibody (8C11).
3
  • Flow
  • IF
  • IHC
  • WB

N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-MSM2
Reactivity:

,

Gene:

Host:

Formalin-fixed, paraffin-embedded human kidney stained with MICA Mouse Recombinant Monoclonal Antibody (MICA/4442). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
5
  • IHC
  • WB

MICA Antibody

Additional information

Catalog Number: 100507436-MSM2
Reactivity:

, , , ,

Gene:

Host:

Formalin-fixed, paraffin-embedded Human Renal Cell Carcinoma Stained with KSP-Cadherin Rabbit Polyclonal Antibody.
0
  • IHC

Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-RBP3
Reactivity:

, ,

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17 / CDH17 Mouse Monoclonal Antibody (CDH17/2618).
4
  • Flow
  • IHC
  • WB

Cadherin 17 / LI Cadherin (Gastric Adenocarcinoma Marker) Antibody

Additional information

Catalog Number: 1015-MSM8
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17 / CDH17 Mouse Monoclonal Antibody (CDH17/2617).
3
  • IHC
  • WB

Cadherin 17 / LI Cadherin (Gastric Adenocarcinoma Marker) Antibody

Additional information

Catalog Number: 1015-MSM7
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with CDH17-Monospecific Mouse Monoclonal Antibody (CDH17/2615).
3
  • IHC
  • WB

Cadherin 17 / LI Cadherin (Gastric Adenocarcinoma Marker) Antibody

Additional information

Catalog Number: 1015-MSM5
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/3874R).
3
  • IHC
  • WB

Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-RBM5
Gene:

Host:

Reactivity:

, , ,

VIEW ALL ANTIBODIES

Other Information

Functions
  • Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Key References
  • Qiu W,Zhou F,Zhang Q,Sun X,Shi X,Liang Y,Wang X,Yue L. Overexpression of nucleolin and different expression sites both related to the prognosis of gastric cancer. APMIS.2013;121(10):919-25.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4691
SwissProt
P19338
Chromosome Location
2q37.1
Mol. Weight of Antigen
76kDa
Alternate Names
Nucleolin, Protein C23, NCL; Nucl; Nucleolin; Protein C23

Summary

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. �This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Worry-Free Guarantee

Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.

Learn More

Need Technical Help?

Our team of scientists are ready to help you with expert recommendations or technical assistance related to applications or product use.

CONTACT US

Follow Us on LinkedIn

NeoBiotechnologies develops and manufactures highly specific, validated monoclonal antibodies for cancer research and diagnostics.

Phone

Whatsapp

Quick Links

Information

© 2026 NeoBiotechnologies.  All Rights Reserved.

Terms and Conditions Privacy Policy
Contact Us