These antibodies perform exceptionally well in the intended applications and have undergone stringent testing and additional validation to ensure monospecificity.
Trial Size Available
Carrier-Free Available
SDS-PAGE Analysis Purified Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R). Confirmation of Integrity and Purity of Antibody.
Formalin-fixed, paraffin-embedded human salivary gland stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R).
Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R).
Western blot analysis of Jurkat cell lysate using Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R).
Formalin-fixed, paraffin-embedded cat liver stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded horse lung stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded mouse stomach stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded cow heart stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
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