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Home >> Antibodies >> NME2 / nm23-H2 / NDPK-B (Suppressor of Metastasis)

NME2 / nm23-H2 / NDPK-B (Suppressor of Metastasis)

Mouse Monoclonal Antibody [Clone CPTC-NME2-2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with NME2 / nm23-H2 Mouse Monoclonal Antibody (CPTC-NME2-2).

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Western Blot Analysis of PC-3 cell lysate using NME2 / nm23-H2 Mouse Monoclonal Antibody (CPTC-NME2-2).

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SDS-PAGE Analysis Purified NME2 / nm23-H2 Mouse Monoclonal Antibody (CPTC-NME2-2). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using NME2 / nm23-H2 / NDPK-B Monoclonal Antibody (CPTC-NME2-2).

Product Details

Synonyms

C myc purine binding transcription factor PUF; C-myc purine-binding transcription factor PUF; epididymis secretory sperm binding protein Li 155an; HEL-S-155an; Histidine protein kinase NDKB;; NDP kinase B; NDPKB; nm23-H2; NM23B; NME/NM23 nucleoside diphosphate kinase 2; nme2; Nucleoside diphosphate kinase B;

Positive Control

PC3, A549, HeLa, Jurkat cells. Ubiquitously expressed in all tissues.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human NME2 protein

Cellular Localization

Cytoplasmic and Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

17kDa

Specificity & Comments

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Tong, Y., et al. 2015. Cancer Lett. 361: 207-217.

Bioinformatics

  •  Entrez Gene ID: 4831
  •  Gene Symbol: NME2
  •  SwissProt: P22392
  •  Chromosome Location: 17q21.33
  •  Unigene: 463456
NBT02248