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Myelin Basic Protein

Recombinant Mouse Monoclonal Antibody [Clone rMBP/4288]

Printable

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Bioinformatics

Formalin-fixed, paraffin-embedded human cerebellum stained with MBP Recombinant Mouse Monoclonal Antibody (rMBP/4288).

Formalin-fixed, paraffin-embedded human cerebellum stained with MBP Recombinant Mouse Monoclonal Antibody (rMBP/4288).

SDS-PAGE Analysis Purified MBP Recombinant Mouse Monoclonal Antibody (rMBP/4288). Confirmation of Purity and Integrity of Antibody

Western Blot Analysis of human brain tissue lysate using MBP Recombinant Mouse Monoclonal Antibody (rMBP/4288).

Formalin-fixed, paraffin-embedded human cerebellum stained with MBP Recombinant Mouse Monoclonal Antibody (rMBP/4288).

SDS-PAGE Analysis Purified MBP Recombinant Mouse Monoclonal Antibody (rMBP/4288). Confirmation of Purity and Integrity of Antibody

Western Blot Analysis of human brain tissue lysate using MBP Recombinant Mouse Monoclonal Antibody (rMBP/4288).

Product Details

Synonyms

GDB; Golli MBP; myelin basic protein; Hemopoietic MBP; HMBPR; HUGO; MLD; Myelin A1 Protein, basic; Myelin Deficient; Myelin membrane encephalitogenic protein; SHI; Shiverer; SP

Positive Control

Human brain or astrocytoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (2-4ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 150-250) of human MBP (exact sequence is proprietary)

Cellular Localization

Cell surface. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

14-22kDa

Specificity & Comments

Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas.