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Home >> Antibodies >> Ki-67 (Proliferating Cell Marker)

Ki-67 (Proliferating Cell Marker)

Mouse Monoclonal Antibody [Clone MKI67/2461]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with Ki67 Mouse Monoclonal Antibody (MKI67/2461).

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Confocal Immunofluorescence of HeLa cells Ki67 Mouse Monoclonal Antibody (MKI67/2461) labeled with CF488 (Green); WGA (Red) is used to label the membrane.

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Flow Cytometric Analysis of HeLa cells using Ki67 Mouse Monoclonal Antibody (MKI67/2461). Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Western Blot Analysis of Raji and Ramos cell lysate using Ki67 Mouse Monoclonal Antibody (MKI67/2461).

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SDS-PAGE Analysis Purified Ki67 Mouse Monoclonal Antibody (MKI67/2461). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Ki67 Mouse Monoclonal Antibody (MKI67/2461).

Product Details

Synonyms

KI-67; Ki67; KI-67 Antigen (KIA); MKI67; Proliferation related Ki-67 antigen

Positive Control

Any actively proliferating cells. Skin, tonsil or lymph node.

Known Applications & Suggested Dilutions

Western Blot, Flow Cyt, IF, IHC-P         More Details

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human Ki67 protein (around aa 2293-2478) (exact sequence is proprietary)

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

345kDa and 395kDa

Specificity & Comments

Ki-67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki-67 index and the histo-pathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki-67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki-67 is a good marker of proliferating cell populations.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Gerdes J, et al. 1984. J. Immunol. 133:1710.

Bioinformatics

  •  Entrez Gene ID: 4288
  •  Gene Symbol: MKI67
  •  SwissProt: P46013
  •  Chromosome Location: 10q26.2
  •  Unigene: 689823
NBT02461