2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

Recombinant Mouse Monoclonal Antibody [Clone rHSPD1/6497]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human kidney stained with HSP60 Recombinant Mouse Monoclonal Antibody (rHSPD1/6497). Inset: 2 Ab only control. Hematoxylin counterstain.

img

Western blot analysis of Hep-2 cell lysate using HSP60 Recombinant Mouse Monoclonal Antibody (rHSPD1/6497).

img

SDS-PAGE Analysis Purified HSP60 Recombinant Mouse Monoclonal Antibody (rHSPD1/6497). Confirmation of Purity and Integrity of Antibody

img

Western blot analysis of Hep-2 and HeLa cell lysate using HSP60 Recombinant Mouse Monoclonal Antibody (rHSPD1/6497).

Product Details

Synonyms

60kDa heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HuCHA60, SPG13, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13

Positive Control

HeLa or Hep2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length HSP60 protein

Cellular Localization

Mitochondria in cytoplasm

Species Reactivity

Human, Mouse, Rat, Hamster, Sheep, Rabbit, Cow, Dog, Pig, Monkey and Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

60kDa

Specificity & Comments

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone rHSPD1/6497, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Boog CJ et al. Two monoclonal antibodies generated against human hsp60 show reactivity with synovial membranes of patients with juvenile chronic arthritis. Journal of Experimental Medicine, 1992, 175(6):1805-10.

Bioinformatics

  •  Entrez Gene ID: 3329
  •  Gene Symbol: HSPD1
  •  SwissProt: P10809
  •  Chromosome Location: 2q33.1
  •  Unigene: 595053