Formalin-fixed, paraffin-embedded human kidney stained with HSP60 Recombinant Mouse Monoclonal Antibody (rHSPD1/6497). Inset: 2° Ab only control. Hematoxylin counterstain.
Western blot analysis of Hep-2 cell lysate using HSP60 Recombinant Mouse Monoclonal Antibody (rHSPD1/6497).
SDS-PAGE Analysis Purified HSP60 Recombinant Mouse Monoclonal Antibody (rHSPD1/6497). Confirmation of Purity and Integrity of Antibody
Western blot analysis of Hep-2 and HeLa cell lysate using HSP60 Recombinant Mouse Monoclonal Antibody (rHSPD1/6497).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone rHSPD1/6497, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.