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Home >> Antibodies >> HLA-B (MHC Class I)

HLA-B (MHC Class I)

Mouse Monoclonal Antibody [Clone JOAN-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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SDS-PAGE Analysis Purified HLA-B Mouse Monoclonal Antibody (JOAN-1). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

HLA-B, HLAB, MHC Class I HLA heavy chain, Leukocyte antigen class I-B

Positive Control

Human peripheral blood lymphocytes, tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Normal human peripheral blood lymphocytes

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG3, kappa

Mol. Weight of Antigen

45kDa

Specificity & Comments

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Young NT et al. Killer cell inhibitory receptor interactions with HLA class I molecules: implications for alloreactivity and transplantation. Hum Immunol 1997, 52(1):1-11
  2. Krensky AM et al Immunomodulation by HLA class I-derived peptides. Transplant Proc 1996, 28(6):3026-8

Bioinformatics

  •  Entrez Gene ID: 3106
  •  Gene Symbol: HLA-B
  •  SwissProt: P01889
  •  Chromosome Location: 6p21.3
  •  Unigene: 654404, 77961
NBT01005