Home >> Antibodies >> Granzyme B (NK/T-Cell Lymphoma Marker)

Granzyme B (NK/T-Cell Lymphoma Marker)

Recombinant Mouse Monoclonal Antibody [Clone rGZMB/4538]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human spleen stained with Granzyme B Recombinant Mouse Monoclonal Antibody (rGZMB/4538).

Formalin-fixed, paraffin-embedded human tonsil stained with Granzyme B Recombinant Mouse Monoclonal Antibody (rGZMB/4538).

Western Blot Analysis of human spleen tissue lysate using Granzyme B Recombinant Mouse Monoclonal Antibody (rGZMB/4538).

SDS-PAGE Analysis Purified GZMB Recombinant Mouse Monoclonal Antibody (rGZMB/4538). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human spleen stained with Granzyme B Recombinant Mouse Monoclonal Antibody (rGZMB/4538).

Formalin-fixed, paraffin-embedded human tonsil stained with Granzyme B Recombinant Mouse Monoclonal Antibody (rGZMB/4538).

Western Blot Analysis of human spleen tissue lysate using Granzyme B Recombinant Mouse Monoclonal Antibody (rGZMB/4538).

SDS-PAGE Analysis Purified GZMB Recombinant Mouse Monoclonal Antibody (rGZMB/4538). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Cathepsin G-like 1; CCPI; CGL1; CSPB; CTLA-1; CTSGL1; Cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; Cytotoxic T-lymphocyte proteinase 2; Fragmentin-2; GRB; Human lymphocyte protein (Hlp); Lymphocyte protease; SECT; T-cell serine protease 1-3E

Positive Control

A431 cells. Human tonsil, spleen or Hodgkin s Lymphoma tissue (IHC).

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human GZMB protein (around aa 73-187) (exact sequence is proprietary)

Cellular Localization

Cytoplasm

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

29-32kDa

Specificity & Comments

Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin s Disease.