Formalin-fixed, paraffin-embedded human spleen stained with Granzyme B Recombinant Rabbit Monoclonal Antibody (GZMB/6530R).

Formalin-fixed, paraffin-embedded human tonsil stained with Granzyme B Recombinant Rabbit Monoclonal Antibody (GZMB/6530R).

SDS-PAGE Analysis Purified GZMB Recombinant Rabbit Monoclonal Antibody (GZMB/6530R). Confirmation of Integrity and Purity of Antibody.

Granzyme B (NK/T-Cell Lymphoma Marker) [GZMB/6530R]

Name: Granzyme B (NK/T-Cell Lymphoma Marker)
SKU: 3002-RBM10
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin s Disease.

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Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Cytolytic granule, Secreted

Gene Name

GZMB

Mol. Weight of Antigen

29-32kDa

Immunogen

Recombinant fragment of human GZMB protein (around aa 73-187) (exact sequence is proprietary)

Database Links

Entrez Gene ID

3002

Unigene

1051

SwissProt

P10144

Additional Information

Clone

GZMB/6530R

Human Gene Symbol

GZMB

Chromosome Location

14q11.2

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Shresta, S., et al. 1995. Natural killer and lymphokine-activated killer cells require granzyme B for the rapid induction of apoptosis in susceptible target cells. Proc. Natl. Acad. Sci. USA 92: 5679-5683.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.