2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker)

gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker)

Mouse Monoclonal Antibody [Clone PMEL/2038]

pdf icon
Catalog No Formulation Size Price (USD)  
6490-MSM8-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
6490-MSM8-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
6490-MSM8-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 429.00
Human Entrez Gene ID 6490
Human SwissProt P40967
Human Unigene 95972
Human Gene Symbol SILV
Human Chromosome Location 12q13.2
Synonyms 95kDa melanocyte-specific secreted glycoprotein, M-beta; Melanocyte lineage specific antigen GP100; Melanocyte protein Pmel 17; Melanoma associated ME20 antigen; Melanosomal matrix protein17; Premelanosome protein (PMEL17); Secreted melanoma-associated ME20 antigen; SILV; Silver homolog
Immunogen Recombinant human SILV protein fragment (around aa 376-502) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 90-100kDa
Cellular Localization Cytoplasmic
Species Reactivity Human. Others-not tested.

Specificity & Comments

Cytotoxic T lymphocytes (CTL s) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.

Known Applications & Suggested Dilutions

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

SK-MEL-28 cells or Melanoma

Supplied As

200ug/ml of Ab purified from bioreactor concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Adema, G.J., et al. 1994. Molecular characterization of the melanocyte lineage-specific antigen gp100. J. Biol. Chem. 269: 20126-20133.
DOC#02038