|Catalog No||Formulation||Size||Price (USD)|
|6490-MSM8-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|6490-MSM8-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||429.00|
|6490-MSM8-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||429.00|
|Human Entrez Gene ID||6490|
|Human Gene Symbol||SILV|
|Human Chromosome Location||12q13.2|
|Synonyms||95kDa melanocyte-specific secreted glycoprotein, M-beta; Melanocyte lineage specific antigen GP100; Melanocyte protein Pmel 17; Melanoma associated ME20 antigen; Melanosomal matrix protein17; Premelanosome protein (PMEL17); Secreted melanoma-associated ME20 antigen; SILV; Silver homolog|
|Immunogen||Recombinant human SILV protein fragment (around aa 376-502) (exact sequence is proprietary)|
|Host / Ig Isotype||Mouse / IgG1, kappa|
|Mol. Weight of Antigen||90-100kDa|
|Species Reactivity||Human. Others-not tested.|
Cytotoxic T lymphocytes (CTL s) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
SK-MEL-28 cells or Melanoma
200ug/ml of Ab purified from bioreactor concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.