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Home >> Antibodies >> gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker)
gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker)

Mouse Monoclonal Antibody [Clone PMEL/2038]

Catalog #
Size
20 ug
100 ug
100 ug
Price (USD)
199.00
429.00
429.00
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Catalog No
Size
20 ug
100 ug
100 ug
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Price (USD)
199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 Mouse Monoclonal Antibody (PMEL/2038).

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Formalin-fixed, paraffin-embedded human Skin stained with gp100 Mouse Monoclonal Antibody (PMEL/2038).

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SDS-PAGE Analysis Purified gp100 Mouse Monoclonal Antibody (PMEL/2038). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using gp100 Mouse Monoclonal Antibody (PMEL/2038).

Product Details

Synonyms

95kDa melanocyte-specific secreted glycoprotein, M-beta; Melanocyte lineage specific antigen GP100; Melanocyte protein Pmel 17; Melanoma associated ME20 antigen; Melanosomal matrix protein17; Premelanosome protein (PMEL17); Secreted melanoma-associated ME20 antigen; SILV; Silver homolog

Positive Control

SK-MEL-28 cells. Melanoma

Known Applications & Suggested Dilutions

ELISA, IHC-P         More Details
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human SILV protein fragment (around aa 376-502) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Others-not tested.

Host / Ig Isotype

Mouse / IgG1, kappa

Specificity & Comments

Cytotoxic T lymphocytes (CTL s) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.

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200ug/ml of Ab purified from bioreactor concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Adema, G.J., et al. 1994. Molecular characterization of the melanocyte lineage-specific antigen gp100. J. Biol. Chem. 269: 20126-20133.

Bioinformatics

  •  Entrez Gene ID: 6490
  •  Gene Symbol: SILV
  •  SwissProt: P40967
  •  Chromosome Location: 12q13.2
  •  Unigene: 95972
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