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Melanosome gp100 / PMEL17 / SILV (Melanoma Marker) Antibody [SPM286]

Additional information

Catalog Number: 6490-MSM2X
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6490-MSM2X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6490-MSM2X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6490-MSM2X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Melanosome gp100 / PMEL17 / SILV (Melanoma Marker) Antibody

Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 Monoclonal Antibody (SPM286).

PMEL Antibody in SDS-PAGE

PMEL Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Melanocyte protein PMEL Mouse Monoclonal Antibody (SPM286). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Horse, Human
Clone
SPM286
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Endoplasmic reticulum membrane, Endosome, Golgi apparatus, Melanosome, Multivesicular body, Secreted
Gene Name
PMEL
Positive Control
SK-MEL-28 cells. Melanoma.
Immunogen
Membranes from a human melanoma metastasis
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
Key References
  • Vennegoor C. et al., Am.J. Pathol. 130: 179-192, 1988.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6490
SwissProt
P40967
Chromosome Location
12q13.2
Mol. Weight of Antigen
90-100kDa
Alternate Names
Melanocyte protein PMEL, ME20-M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, 95kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog

Summary

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

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