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Home >> Antibodies >> gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker)

gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker)

Mouse Monoclonal Antibody [Clone HMB45 + PMEL/783]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 / Melanosome Monoclonal Antibody (HMB45 + PMEL/783).

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Formalin-fixed, paraffin-embedded human Testis stained with gp100 / Melanosome Monoclonal Antibody (HMB45 + PMEL/783).

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Western Blot Analysis of COLO-38 cell lysate using gp100 / Melanosome MAb (HMB45 + PMEL/783).

Product Details

Synonyms

95kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog

Positive Control

CACO-38 or SK-MEL-28 cells. Melanoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (2-4ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Extract of pigmented melanoma metastases from lymph nodes (HMB45); Recombinant human SILV protein (PMEL/783)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa + Mouse / IgG1, kappa

Mol. Weight of Antigen

26-100kDa

Specificity & Comments

The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Gown AM, et. al. American Journal of Pathology, 1986, 123(2):195-203.

Bioinformatics

  •  Entrez Gene ID: 6490
  •  Gene Symbol: SILV
  •  SwissProt: P40967
  •  Chromosome Location: 12q13.2
  •  Unigene: 95972
NBT00951