Formalin-fixed, paraffin-embedded human Schwanoma stained with GFAP Monoclonal Antibody (SPM248).
Western Blot Analysis of human brain tissue lysate using GFAP Monoclonal Antibody (SPM248).
SDS-PAGE Analysis Purified GFAP Monoclonal Antibody (SPM248). Confirmation of Integrity and Purity of Antibody.
Flow Cytometric Analysis of T98G cells using GFAP Mouse Monoclonal Antibody (SPM248) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.