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Home >> Antibodies >> GFAP (Astrocyte & Neural Stem Cell Marker)

GFAP (Astrocyte & Neural Stem Cell Marker)

Mouse Monoclonal Antibody [Clone GFAP/6879]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP Mouse Monoclonal Antibody (GFAP/6879). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP Mouse Monoclonal Antibody (GFAP/6879).

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Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP Mouse Monoclonal Antibody (GFAP/6879).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to GFAP (GFAP/6879).

Product Details

Synonyms

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Positive Control

Human brain or astrocytoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 150-250) of human GFAP protein (exact sequence is proprietary)

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2c, kappa

Mol. Weight of Antigen

~50kDa

Specificity & Comments

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. McLendon, R.E. and Bigner, D.D. 1994. Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations. Brain Pathol. 4: 221-228.

Bioinformatics

  •  Entrez Gene ID: 2670
  •  Gene Symbol: GFAP
  •  SwissProt: P14136
  •  Chromosome Location: 17q21.31
  •  Unigene: 514227