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Home >> Antibodies >> ETS2 (Transcription Factor)

ETS2 (Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-ETS2-1D9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Immunofluorescence Analysis of PFA-fixed HeLa cells stained using ETS2 Mouse Monoclonal Antibody (PCRP-ETS2-1D9) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

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SDS-PAGE Analysis Purified ETS2 Mouse Monoclonal Antibody (PCRP-ETS2-1D9). Confirmation of Purity and Integrity of Antibody.

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Flow cytometric analysis of PFA-fixed HeLa cells. ETS2 Mouse Monoclonal Antibody (PCRP-ETS2-1D9) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using ETS2-Monospecific Mouse Monoclonal Antibody (PCRP-ETS2-1D9).

Product Details

Synonyms

C ets 2 protein; ETS2; ETS2IT1; Oncogene ETS2; Protein C-ets-2; v ets avian erythroblastosis virus E2 oncogene homolog 2; V ets avian erythroblastosis virus E26 oncogene homolog 2

Positive Control

Human thymus, lung or cerebellum.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF, IP         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human ETS2 protein

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

52kDa

Specificity & Comments

ETS2 (or Reln) is a large glycoprotein that is secreted by Cajal-Retzius cells in the forebrain and by granule neurons in the cerebellum. ETS2 was shown to be mutated in reeler mice, a mutation that is associated with widespread disruption of laminated regions of the brain, leading to impaired motor coordination, tremors and ataxia. ETS2 protein expression is complex and changes throughout development. ETS2 appears to function upstream of Dab1, in a signaling pathway that controls cell positioning in the developing brain, and is also thought to be a direct effector of the neurotrophin BDNF.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Goffinet AM, et al. The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

Bioinformatics

  •  Entrez Gene ID: 2114
  •  Gene Symbol: ETS2
  •  SwissProt: P15036
  •  Chromosome Location: 21q22.2
  •  Unigene: 644231