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Home >> Antibodies >> E-Cadherin(CDH1) / CD324 (Intercellular Junction Marker)

E-Cadherin(CDH1) / CD324 (Intercellular Junction Marker)

Recombinant Rabbit Monoclonal Antibody [Clone CDH1/4398R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human cervix stained with E-Cadherin Rabbit RecombinantMonoclonal Antibody (CDH1/4398R).

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Formalin-fixed, paraffin-embedded human stomach stained with E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/4398R).

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IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using CDH1/4398R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Formalin-fixed, paraffin-embedded human cervix stained with E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/4398R).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/4398R).

Product Details

Synonyms

Arc 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD324; CDH1; CDHE; E-Cad/CTF3; ECAD; Epithelial cadherin; epithelial calcium dependent adhesion protein; Liver cell adhesion molecule (LCAM); Uvomorulin (UVO)

Positive Control

MCF-7 or SK-BR3 cells.Prostate or colon carcinomas.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Synthetic peptide corresponding to E-Cadherin residues within aa600-700 of E-cadherin

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

120-80kDa (Mature); 135kDa (Precursor)

Specificity & Comments

E-cadherin is a transmembrane, calcium dependent cell adhesion protein that mediates cell to cell adhesion and maintains structural and functional integrity of epithelial tissues. It also has pivotal barrier functions and maintains the polarity of epithelial cells. Reduced or aberrant E-cadherin expression breaks cell to cell contacts, and thus, cells acquire the ability to migrate. In normal tissues, immunostaining of E-cadherin is localized to the membrane of epithelial cells, consistent with its role in cell adhesion. And in tumor tissues, E-cadherin stains positively in glandular epithelium as well as adenocarcinomas of the lung, gastrointestinal tract, and ovary. It has also been shown to be positive in some thyroid carcinomas. A combination of E-cadherin and p120 catenin may help distinguish ductal carcinoma of the breast from lobular carcinoma. And also, several studies have reported that reduced E-cadherin expression is correlated with poor prognosis in several types of carcinomas.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Umbas, R., et al. 1992. Expression of the cellular adhesion molecule E-cadherin is reduced or absent in high-grade prostate cancer. Cancer Res. 52: 5104-5109.

Bioinformatics

  •  Entrez Gene ID: 999
  •  Gene Symbol: CDH1
  •  SwissProt: P12830
  •  Chromosome Location: 16q22.1
  •  Unigene: 461086