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Home >> Antibodies >> Cytokeratin 6 (Basal Cell Marker)

Cytokeratin 6 (Basal Cell Marker)

Mouse Monoclonal Antibody [Clone SPM269]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Cytokeratin 6 Mouse Monoclonal Antibody (SPM269).

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Immunofluorescence Analysis of RAW cells labeling KRT6 with KRT6 Mouse Monoclonal Antibody (SPM269) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Reddot (Red).

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Flow Cytometric Analysis of RAW cells using Cytokeratin 6 Mouse Monoclonal Antibody (SPM269) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

CK6A, CK6B, CK6C, CK6D, CK6E, Keratin Type II Cytoskeletal 6A, Keratin Type II Cytoskeletal 6B, Keratin Type II Cytoskeletal 6C, Keratin Type II Cytoskeletal 6D, Keratin Type II Cytoskeletal 6E, KRT6, KRT6A, KRT6B, KRT6C, KRT6D, KRT6E

Positive Control

RAW, HeLa, Suprabasal cells of the outer hair follicle root sheath in skin. Basal cell carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with pepsin (Sigma Cat. #P-7012) at 1mg/ml Tris-HCl, pH 2.0 for 10-15 min at RT)Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from C-terminus of human keratin 6

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

56kDa

Specificity & Comments

This MAb recognizes a protein of 56kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wetzels RH, et. al. American Journal of Pathology, 1991, 138(3):751-63.

Bioinformatics

NBT00675