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Home >> Antibodies >> Cytokeratin 18 (KRT18)

Cytokeratin 18 (KRT18)

Mouse Monoclonal Antibody [Clone KRT18/1190]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

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Formalin-fixed, paraffin-embedded Rat Colon stained with Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

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Western Blot Analysis of human A) Stomach B) Intestine lysate using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

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Confocal immunofluorescence image of HeLa cells using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190). Green (CF488). Reddot is used to label nuclei Red.

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Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

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Western Blot Analysis of human A549 and HEP-2 cell lysate using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

Product Details

Synonyms

Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18

Positive Control

A549, HEP-2, MCF-7, HeLa cells, Breast Cancer.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human KRT18 protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

45kDa

Specificity & Comments

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Taylor -Papadimitriou et.al. J.Cell Sci. 94: 403-13 (1989).
  2. Vojtek et al. Folia Biol. 35(6): 373-382 (1989).

Bioinformatics

  •  Entrez Gene ID: 3875
  •  Gene Symbol: KRT18
  •  SwissProt: P05783
  •  Chromosome Location: 12q13.13
  •  Unigene: 406013
NBT01190