Home >> Antibodies >> Cytokeratin 18 (KRT18)

Cytokeratin 18 (KRT18)

Mouse Monoclonal Antibody [Clone KRT18/1190]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

Formalin-fixed, paraffin-embedded rat colon stained with Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

Western blot analysis of human A) stomach B) intestine tissue lysates using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

Confocal immunofluorescence analysis of MeOH-fixed HeLa cells. Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot

Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

Western Blot Analysis of human A549 and HEP-2 cell lysates using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

Formalin-fixed, paraffin-embedded rat colon stained with Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

Western blot analysis of human A) stomach B) intestine tissue lysates using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

Confocal immunofluorescence analysis of MeOH-fixed HeLa cells. Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot

Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

Western Blot Analysis of human A549 and HEP-2 cell lysates using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).

Product Details

Synonyms

Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18 Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18

Positive Control

A549, HEP-2, MCF-7 or HeLa cells. Human breast or prostate cancer.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human KRT18 protein

Cellular Localization

Cytoplasm.

Species Reactivity

Human. Dog. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

45kDa

Specificity & Comments

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.