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Home >> Antibodies >> Cytokeratin 14 (Squamous Cell Marker)

Cytokeratin 14 (Squamous Cell Marker)

Mouse Monoclonal Antibody [Clone SPM263]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Monoclonal Antibody (SPM263).

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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Monoclonal Antibody (SPM263).

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SDS-PAGE Analysis Purified Cytokeratin 14 Monoclonal Antibody (SPM263). Confirmation of Integrity and Purity of Antibody.

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Immunofluorescence Analysis of A549 cells labeling KRT14 with Cytokeratin 14 MAb (SPM263) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Product Details

Synonyms

CK-14; Dowling Meara; ebs3; ebs4; Epidermolysis Bullosa Simplex; k14; Keratin 14; Keratin Type I Cytoskeletal 14; Koebner; krt14; NFJ

Positive Control

A431 cells. Skin or Squamous cell carcinoma.

Known Applications & Suggested Dilutions

IF, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14.

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG3, kappa

Mol. Weight of Antigen

50kDa

Specificity & Comments

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Perkins W et. al. J Cutaneous Pathol, 1992, 19(6):476-82.
  2. Kasper M. Histochemistry, 1991, 95(6):613-20.
  3. Leigh I M et. al. (1988) Dermatology, 91:415.

Bioinformatics

  •  Entrez Gene ID: 3861
  •  Gene Symbol: KRT14
  •  SwissProt: P02533
  •  Chromosome Location: 17q21.2
  •  Unigene: 6545380
NBT00499