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Cytokeratin 14 (Squamous Cell Marker) Antibody [SPM263]

Additional information

Catalog Number: 3861-MSM1X
Reactivity:

, ,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3861-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3861-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3861-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin 14 (Squamous Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Monoclonal Antibody (SPM263).

Cytokeratin 14 (Squamous Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Monoclonal Antibody (SPM263).

Cytokeratin 14 (Squamous Cell Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified Cytokeratin 14 Monoclonal Antibody (SPM263). Confirmation of Integrity and Purity of Antibody.

Cytokeratin 14 (Squamous Cell Marker) Antibody - Image 4

Immunofluorescence Analysis of A549 cells labeling KRT14 with Cytokeratin 14 MAb (SPM263) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Mouse, Rat
Clone
SPM263
Isotype
IgG3
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
KRT14
Positive Control
A431 cells. Skin or Squamous cell carcinoma.
Immunogen
A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14.
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Key References
  • Perkins W et. al. J Cutaneous Pathol, 1992, 19(6):476-82.
  • Kasper M. Histochemistry, 1991, 95(6):613-20.
  • Leigh I M et. al. (1988) Dermatology, 91:415.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3861
SwissProt
P02533
Chromosome Location
17q21.2
Mol. Weight of Antigen
~50kDa
Alternate Names
Keratin, type I cytoskeletal 14, Cytokeratin-14, Keratin-14, CK-14; Dowling Meara; ebs3; ebs4; Epidermolysis Bullosa Simplex; k14; Keratin 14; Keratin Type I Cytoskeletal 14; Koebner; krt14; NFJ

Summary

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

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